Loss of corneal Langerhans cells during storage in organ culture medium, Optisol and McCarey-Kaufman medium
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چکیده
منابع مشابه
Efficacy and safety of antifungal additives in Optisol-GS corneal storage medium.
IMPORTANCE Optisol-GS, the most common corneal storage medium in the United States, contains antibacterial but no antifungal supplementation. Most postkeratoplasty endophthalmitis and keratitis cases are now of a fungal origin. OBJECTIVE To assess the efficacy and safety of voriconazole and amphotericin B in reducing Candida species contamination of Optisol-GS under normal storage conditions....
متن کاملCorneal temperature reversal after storage in Chen medium compared with Optisol GS.
PURPOSE To compare corneal endothelial cell function by measuring corneal thickness during temperature reversal between corneas stored in two different storage media, Optisol GS and Chen Medium (CM). METHODS Twenty paired corneas from 10 human donors were randomly assigned for storage at 4 degrees C in Optisol GS (10 corneas) or CM (10 corneas). The storage media were masked, and measurements...
متن کاملA randomized, double-masked clinical trial of Optisol-GS vs Chen Medium for human corneal storage.
OBJECTIVE To determine whether the clinical outcome of corneal transplantation performed with tissue stored in Chen Medium (CM; Chen Laboratories, Phoenix, Md) is superior to that of Optisol-GS (Chiron Ophthalmics, Irvine, Calif). DESIGN Randomized, double-masked trial. PARTICIPANTS AND SETTING Ninety patients undergoing corneal transplantation at a tertiary center. INTERVENTION Donor cor...
متن کاملOxidative stress gradient in a medium during human corneal organ culture
PURPOSE Lipid peroxidation content was measured in an organ culture medium after one-week storage of human donor corneas. Moreover, the effects of the medium on oxidative stress, antioxidant capacity, and the proliferation of cultured human corneal cells were studied. METHODS The medium was sampled from the upper and lower halves of storage vials and from controls (n=42). Malondialdehyde (MDA...
متن کاملEffects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium.
PURPOSE A previous report has described the use of eye bank storage of cultured human limbal epithelial cells (HLECs) to provide a reliable source of tissue for treating limbal stem cell deficiency. In the present study, conventional organ culture (OC) storage and Optisol-GS (Bausch & Lomb, Irvine, CA) storage of cultured HLECs were compared. METHODS Three-week HLEC cultures were either organ...
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ژورنال
عنوان ژورنال: Eye
سال: 1998
ISSN: 0950-222X,1476-5454
DOI: 10.1038/eye.1998.22